invitrogen cellmask deep red (Thermo Fisher)
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Invitrogen Cellmask Deep Red, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 97 stars, based on 1 article reviews
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Staining:Article Title: Fabrication of cytotoxic mirror image nanopores. Article Snippet: .. After 24 h of treatment, cells were stained with Article Title: TANGO2 binds crystallin alpha B and its loss causes desminopathy. Article Snippet: .. Control and TANGO2–/– cells were cultured for 48 h in 35mm Ibidi 500-grid plastic-bottomed μ-dishes before staining with 50 nM MitoTracker Orange (5 min), and primary fixation with phosphatebuffered 4% paraformaldehyde at 37 °C for 1 h. The fixed samples were post stained ( Article Title: Dopamine-driven increase in IL-1β in myeloid cells is mediated by differential dopamine receptor expression and exacerbated by HIV. Article Snippet: After the primary incubation, iMicroglia were incubated with an Alexa Fluor 488 secondary antibody (A-11001, Fisher Scientific) prepared in blocking buffer for 1 h at room temperature. .. Afterwards, all cells were stained with Article Title: TANGO2 binds crystallin alpha B and its loss causes desminopathy Article Snippet: .. Control and TANGO2 –/– cells were cultured for 48 h in 35 mm Ibidi 500-grid plastic-bottomed μ-dishes before staining with 50 nM MitoTracker Orange (5 min), and primary fixation with phosphate-buffered 4% paraformaldehyde at 37 °C for 1 h. The fixed samples were post stained (30 min) with 2.5 mg/ml Article Title: Large-Scale Functional Characterization of Low-Density Lipoprotein Receptor Gene Variants Improves Risk Assessment in Cardiovascular Disease. Article Snippet: .. Fluorescent low-density lipoprotein (DiI-LDL) uptake studies were performed similar to previous studies,15 with the following modifications: following 72 hours of overall treatment, DiI-LDL uptake was stimulated by adding 10 mg/mL DiI-LDL solution and incubated for 30 minutes at 37 C. Subsequently, cells were rinsed with phosphate-buffered saline, fixed with 4% paraformaldehyde, washed, and stained with Hoechst 33342 (5 mg/mL) and Article Title: Fabrication of cytotoxic mirror image nanopores Article Snippet: .. After 24 h of treatment, cells were stained with Control:Article Title: TANGO2 binds crystallin alpha B and its loss causes desminopathy. Article Snippet: .. Control and TANGO2–/– cells were cultured for 48 h in 35mm Ibidi 500-grid plastic-bottomed μ-dishes before staining with 50 nM MitoTracker Orange (5 min), and primary fixation with phosphatebuffered 4% paraformaldehyde at 37 °C for 1 h. The fixed samples were post stained ( Article Title: TANGO2 binds crystallin alpha B and its loss causes desminopathy Article Snippet: .. Control and TANGO2 –/– cells were cultured for 48 h in 35 mm Ibidi 500-grid plastic-bottomed μ-dishes before staining with 50 nM MitoTracker Orange (5 min), and primary fixation with phosphate-buffered 4% paraformaldehyde at 37 °C for 1 h. The fixed samples were post stained (30 min) with 2.5 mg/ml Cell Culture:Article Title: TANGO2 binds crystallin alpha B and its loss causes desminopathy. Article Snippet: .. Control and TANGO2–/– cells were cultured for 48 h in 35mm Ibidi 500-grid plastic-bottomed μ-dishes before staining with 50 nM MitoTracker Orange (5 min), and primary fixation with phosphatebuffered 4% paraformaldehyde at 37 °C for 1 h. The fixed samples were post stained ( Article Title: TANGO2 binds crystallin alpha B and its loss causes desminopathy Article Snippet: .. Control and TANGO2 –/– cells were cultured for 48 h in 35 mm Ibidi 500-grid plastic-bottomed μ-dishes before staining with 50 nM MitoTracker Orange (5 min), and primary fixation with phosphate-buffered 4% paraformaldehyde at 37 °C for 1 h. The fixed samples were post stained (30 min) with 2.5 mg/ml Imaging:Article Title: TANGO2 binds crystallin alpha B and its loss causes desminopathy. Article Snippet: .. Control and TANGO2–/– cells were cultured for 48 h in 35mm Ibidi 500-grid plastic-bottomed μ-dishes before staining with 50 nM MitoTracker Orange (5 min), and primary fixation with phosphatebuffered 4% paraformaldehyde at 37 °C for 1 h. The fixed samples were post stained ( Article Title: TANGO2 binds crystallin alpha B and its loss causes desminopathy Article Snippet: .. Control and TANGO2 –/– cells were cultured for 48 h in 35 mm Ibidi 500-grid plastic-bottomed μ-dishes before staining with 50 nM MitoTracker Orange (5 min), and primary fixation with phosphate-buffered 4% paraformaldehyde at 37 °C for 1 h. The fixed samples were post stained (30 min) with 2.5 mg/ml Microscopy:Article Title: TANGO2 binds crystallin alpha B and its loss causes desminopathy. Article Snippet: .. Control and TANGO2–/– cells were cultured for 48 h in 35mm Ibidi 500-grid plastic-bottomed μ-dishes before staining with 50 nM MitoTracker Orange (5 min), and primary fixation with phosphatebuffered 4% paraformaldehyde at 37 °C for 1 h. The fixed samples were post stained ( Article Title: TANGO2 binds crystallin alpha B and its loss causes desminopathy Article Snippet: .. Control and TANGO2 –/– cells were cultured for 48 h in 35 mm Ibidi 500-grid plastic-bottomed μ-dishes before staining with 50 nM MitoTracker Orange (5 min), and primary fixation with phosphate-buffered 4% paraformaldehyde at 37 °C for 1 h. The fixed samples were post stained (30 min) with 2.5 mg/ml Laser-Scanning Microscopy:Article Title: Dopamine-driven increase in IL-1β in myeloid cells is mediated by differential dopamine receptor expression and exacerbated by HIV. Article Snippet: After the primary incubation, iMicroglia were incubated with an Alexa Fluor 488 secondary antibody (A-11001, Fisher Scientific) prepared in blocking buffer for 1 h at room temperature. .. Afterwards, all cells were stained with Saline:Article Title: Modular Calcium‐Responsive and CD9‐Targeted Phospholipase System Enhancing Endosomal Escape for DNA Delivery Article Snippet: Dithiothreitol (DTT), Halt protease‐inhibitor‐cocktail, polyethyleneimine (PEI), imidazole, heparin agarose, KCl, Tris‐base, sodium dodecyl sulfate (SDS), 2‐mercaptoethanol, 4‐(2‐hydroxyethyl)‐1‐piperazineethanesulfonic acid (HEPES), agarose, kanamycin sulfate, glucose, Hoechst 33342, and Amicon Ultra‐15 PLGC Ultracel‐PL Membrane were purchased from Sigma‐Aldrich (St. Louis, MO, USA). .. Phosphate‐buffered saline (PBS) (1 mM KH 2 PO 4 , 155 mM NaCl, 3 mM Na 2 HPO 4 ), FBS, Dulbecco's Modified Eagle Medium (DMEM) GlutaMAX, penicillin‐streptomycin (pen‐strep), LP3000, Article Title: Large-Scale Functional Characterization of Low-Density Lipoprotein Receptor Gene Variants Improves Risk Assessment in Cardiovascular Disease. Article Snippet: .. Fluorescent low-density lipoprotein (DiI-LDL) uptake studies were performed similar to previous studies,15 with the following modifications: following 72 hours of overall treatment, DiI-LDL uptake was stimulated by adding 10 mg/mL DiI-LDL solution and incubated for 30 minutes at 37 C. Subsequently, cells were rinsed with phosphate-buffered saline, fixed with 4% paraformaldehyde, washed, and stained with Hoechst 33342 (5 mg/mL) and Modification:Article Title: Modular Calcium‐Responsive and CD9‐Targeted Phospholipase System Enhancing Endosomal Escape for DNA Delivery Article Snippet: Dithiothreitol (DTT), Halt protease‐inhibitor‐cocktail, polyethyleneimine (PEI), imidazole, heparin agarose, KCl, Tris‐base, sodium dodecyl sulfate (SDS), 2‐mercaptoethanol, 4‐(2‐hydroxyethyl)‐1‐piperazineethanesulfonic acid (HEPES), agarose, kanamycin sulfate, glucose, Hoechst 33342, and Amicon Ultra‐15 PLGC Ultracel‐PL Membrane were purchased from Sigma‐Aldrich (St. Louis, MO, USA). .. Phosphate‐buffered saline (PBS) (1 mM KH 2 PO 4 , 155 mM NaCl, 3 mM Na 2 HPO 4 ), FBS, Dulbecco's Modified Eagle Medium (DMEM) GlutaMAX, penicillin‐streptomycin (pen‐strep), LP3000, Incubation:Article Title: Large-Scale Functional Characterization of Low-Density Lipoprotein Receptor Gene Variants Improves Risk Assessment in Cardiovascular Disease. Article Snippet: .. Fluorescent low-density lipoprotein (DiI-LDL) uptake studies were performed similar to previous studies,15 with the following modifications: following 72 hours of overall treatment, DiI-LDL uptake was stimulated by adding 10 mg/mL DiI-LDL solution and incubated for 30 minutes at 37 C. Subsequently, cells were rinsed with phosphate-buffered saline, fixed with 4% paraformaldehyde, washed, and stained with Hoechst 33342 (5 mg/mL) and |
