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invitrogen cellmask deep red  (Thermo Fisher)


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    Structured Review

    Thermo Fisher invitrogen cellmask deep red
    Invitrogen Cellmask Deep Red, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cellmask+deep+red/CryoPlus+Storage+Systems/pmc13045418-209-8-8
    Average 97 stars, based on 1 article reviews
    invitrogen cellmask deep red - by Bioz Stars, 2026-09
    97/100 stars

    Images

    Related Articles

    Staining:

    Article Title: Fabrication of cytotoxic mirror image nanopores.
    Article Snippet: .. After 24 h of treatment, cells were stained with CellMask Deep Red (Thermo Fisher Scientific, C10046; 1:4000 dilution in PBS) for 5min at 37 °C. .. After membrane staining, the cells were washed with PBS and fixed in 2% PFA (Himedia, TCL119) for 5min.

    Article Title: TANGO2 binds crystallin alpha B and its loss causes desminopathy.
    Article Snippet: .. Control and TANGO2–/– cells were cultured for 48 h in 35mm Ibidi 500-grid plastic-bottomed μ-dishes before staining with 50 nM MitoTracker Orange (5 min), and primary fixation with phosphatebuffered 4% paraformaldehyde at 37 °C for 1 h. The fixed samples were post stained (30min) with 2.5 mg/ml CellMask Deep Red (Thermo Fisher Scientific) and 1 μgml−1 Hoechst 33342 (Merck) for room temperature imaging on a Nikon A1 confocal microscope equipped with a ×40, 1.15 numerical aperture objective (water immersion; PlanApo Lambda DIC N2, Nikon). ..

    Article Title: Dopamine-driven increase in IL-1β in myeloid cells is mediated by differential dopamine receptor expression and exacerbated by HIV.
    Article Snippet: After the primary incubation, iMicroglia were incubated with an Alexa Fluor 488 secondary antibody (A-11001, Fisher Scientific) prepared in blocking buffer for 1 h at room temperature. .. Afterwards, all cells were stained with CellMask Deep Red (250 ng/mL, catalog # C10046 Thermo Fisher) for 10 min and cover slipped with SlowFadeTM Glass Soft-set Antifade Mountant, with DAPI (catalog # S36920 Thermo Fisher). iMicroglia images were acquired at 60X using an Olympus FV3000 confocal laser scanning microscope. .. For Western blot analysis, hMDM were cultured at 9.5 × 105 cells per well in 6-well plates (Thermo Fisher).

    Article Title: TANGO2 binds crystallin alpha B and its loss causes desminopathy
    Article Snippet: .. Control and TANGO2 –/– cells were cultured for 48 h in 35 mm Ibidi 500-grid plastic-bottomed μ-dishes before staining with 50 nM MitoTracker Orange (5 min), and primary fixation with phosphate-buffered 4% paraformaldehyde at 37 °C for 1 h. The fixed samples were post stained (30 min) with 2.5 mg/ml CellMask Deep Red (Thermo Fisher Scientific) and 1 μg ml −1 Hoechst 33342 (Merck) for room temperature imaging on a Nikon A1 confocal microscope equipped with a ×40, 1.15 numerical aperture objective (water immersion; PlanApo Lambda DIC N2, Nikon). ..

    Article Title: Large-Scale Functional Characterization of Low-Density Lipoprotein Receptor Gene Variants Improves Risk Assessment in Cardiovascular Disease.
    Article Snippet: .. Fluorescent low-density lipoprotein (DiI-LDL) uptake studies were performed similar to previous studies,15 with the following modifications: following 72 hours of overall treatment, DiI-LDL uptake was stimulated by adding 10 mg/mL DiI-LDL solution and incubated for 30 minutes at 37 C. Subsequently, cells were rinsed with phosphate-buffered saline, fixed with 4% paraformaldehyde, washed, and stained with Hoechst 33342 (5 mg/mL) and CellMask Deep Red (0.5 mg/mL) (Thermo Fisher Scientific). .. Images were acquired using a PerkinElmer OperaPhenix microscope equipped with 20 or 40 objectives.

    Article Title: Fabrication of cytotoxic mirror image nanopores
    Article Snippet: .. After 24 h of treatment, cells were stained with CellMask Deep Red (Thermo Fisher Scientific, C10046 ; 1:4000 dilution in PBS) for 5 min at 37 °C. .. After membrane staining, the cells were washed with PBS and fixed in 2% PFA (Himedia, TCL119) for 5 min. After PBS wash, the coverslips were carefully removed from the wells and mounted with ProLongTM Gold Antifade Mountant (Invitrogen, P10144 ).

    Control:

    Article Title: TANGO2 binds crystallin alpha B and its loss causes desminopathy.
    Article Snippet: .. Control and TANGO2–/– cells were cultured for 48 h in 35mm Ibidi 500-grid plastic-bottomed μ-dishes before staining with 50 nM MitoTracker Orange (5 min), and primary fixation with phosphatebuffered 4% paraformaldehyde at 37 °C for 1 h. The fixed samples were post stained (30min) with 2.5 mg/ml CellMask Deep Red (Thermo Fisher Scientific) and 1 μgml−1 Hoechst 33342 (Merck) for room temperature imaging on a Nikon A1 confocal microscope equipped with a ×40, 1.15 numerical aperture objective (water immersion; PlanApo Lambda DIC N2, Nikon). ..

    Article Title: TANGO2 binds crystallin alpha B and its loss causes desminopathy
    Article Snippet: .. Control and TANGO2 –/– cells were cultured for 48 h in 35 mm Ibidi 500-grid plastic-bottomed μ-dishes before staining with 50 nM MitoTracker Orange (5 min), and primary fixation with phosphate-buffered 4% paraformaldehyde at 37 °C for 1 h. The fixed samples were post stained (30 min) with 2.5 mg/ml CellMask Deep Red (Thermo Fisher Scientific) and 1 μg ml −1 Hoechst 33342 (Merck) for room temperature imaging on a Nikon A1 confocal microscope equipped with a ×40, 1.15 numerical aperture objective (water immersion; PlanApo Lambda DIC N2, Nikon). ..

    Cell Culture:

    Article Title: TANGO2 binds crystallin alpha B and its loss causes desminopathy.
    Article Snippet: .. Control and TANGO2–/– cells were cultured for 48 h in 35mm Ibidi 500-grid plastic-bottomed μ-dishes before staining with 50 nM MitoTracker Orange (5 min), and primary fixation with phosphatebuffered 4% paraformaldehyde at 37 °C for 1 h. The fixed samples were post stained (30min) with 2.5 mg/ml CellMask Deep Red (Thermo Fisher Scientific) and 1 μgml−1 Hoechst 33342 (Merck) for room temperature imaging on a Nikon A1 confocal microscope equipped with a ×40, 1.15 numerical aperture objective (water immersion; PlanApo Lambda DIC N2, Nikon). ..

    Article Title: TANGO2 binds crystallin alpha B and its loss causes desminopathy
    Article Snippet: .. Control and TANGO2 –/– cells were cultured for 48 h in 35 mm Ibidi 500-grid plastic-bottomed μ-dishes before staining with 50 nM MitoTracker Orange (5 min), and primary fixation with phosphate-buffered 4% paraformaldehyde at 37 °C for 1 h. The fixed samples were post stained (30 min) with 2.5 mg/ml CellMask Deep Red (Thermo Fisher Scientific) and 1 μg ml −1 Hoechst 33342 (Merck) for room temperature imaging on a Nikon A1 confocal microscope equipped with a ×40, 1.15 numerical aperture objective (water immersion; PlanApo Lambda DIC N2, Nikon). ..

    Imaging:

    Article Title: TANGO2 binds crystallin alpha B and its loss causes desminopathy.
    Article Snippet: .. Control and TANGO2–/– cells were cultured for 48 h in 35mm Ibidi 500-grid plastic-bottomed μ-dishes before staining with 50 nM MitoTracker Orange (5 min), and primary fixation with phosphatebuffered 4% paraformaldehyde at 37 °C for 1 h. The fixed samples were post stained (30min) with 2.5 mg/ml CellMask Deep Red (Thermo Fisher Scientific) and 1 μgml−1 Hoechst 33342 (Merck) for room temperature imaging on a Nikon A1 confocal microscope equipped with a ×40, 1.15 numerical aperture objective (water immersion; PlanApo Lambda DIC N2, Nikon). ..

    Article Title: TANGO2 binds crystallin alpha B and its loss causes desminopathy
    Article Snippet: .. Control and TANGO2 –/– cells were cultured for 48 h in 35 mm Ibidi 500-grid plastic-bottomed μ-dishes before staining with 50 nM MitoTracker Orange (5 min), and primary fixation with phosphate-buffered 4% paraformaldehyde at 37 °C for 1 h. The fixed samples were post stained (30 min) with 2.5 mg/ml CellMask Deep Red (Thermo Fisher Scientific) and 1 μg ml −1 Hoechst 33342 (Merck) for room temperature imaging on a Nikon A1 confocal microscope equipped with a ×40, 1.15 numerical aperture objective (water immersion; PlanApo Lambda DIC N2, Nikon). ..

    Microscopy:

    Article Title: TANGO2 binds crystallin alpha B and its loss causes desminopathy.
    Article Snippet: .. Control and TANGO2–/– cells were cultured for 48 h in 35mm Ibidi 500-grid plastic-bottomed μ-dishes before staining with 50 nM MitoTracker Orange (5 min), and primary fixation with phosphatebuffered 4% paraformaldehyde at 37 °C for 1 h. The fixed samples were post stained (30min) with 2.5 mg/ml CellMask Deep Red (Thermo Fisher Scientific) and 1 μgml−1 Hoechst 33342 (Merck) for room temperature imaging on a Nikon A1 confocal microscope equipped with a ×40, 1.15 numerical aperture objective (water immersion; PlanApo Lambda DIC N2, Nikon). ..

    Article Title: TANGO2 binds crystallin alpha B and its loss causes desminopathy
    Article Snippet: .. Control and TANGO2 –/– cells were cultured for 48 h in 35 mm Ibidi 500-grid plastic-bottomed μ-dishes before staining with 50 nM MitoTracker Orange (5 min), and primary fixation with phosphate-buffered 4% paraformaldehyde at 37 °C for 1 h. The fixed samples were post stained (30 min) with 2.5 mg/ml CellMask Deep Red (Thermo Fisher Scientific) and 1 μg ml −1 Hoechst 33342 (Merck) for room temperature imaging on a Nikon A1 confocal microscope equipped with a ×40, 1.15 numerical aperture objective (water immersion; PlanApo Lambda DIC N2, Nikon). ..

    Laser-Scanning Microscopy:

    Article Title: Dopamine-driven increase in IL-1β in myeloid cells is mediated by differential dopamine receptor expression and exacerbated by HIV.
    Article Snippet: After the primary incubation, iMicroglia were incubated with an Alexa Fluor 488 secondary antibody (A-11001, Fisher Scientific) prepared in blocking buffer for 1 h at room temperature. .. Afterwards, all cells were stained with CellMask Deep Red (250 ng/mL, catalog # C10046 Thermo Fisher) for 10 min and cover slipped with SlowFadeTM Glass Soft-set Antifade Mountant, with DAPI (catalog # S36920 Thermo Fisher). iMicroglia images were acquired at 60X using an Olympus FV3000 confocal laser scanning microscope. .. For Western blot analysis, hMDM were cultured at 9.5 × 105 cells per well in 6-well plates (Thermo Fisher).

    Saline:

    Article Title: Modular Calcium‐Responsive and CD9‐Targeted Phospholipase System Enhancing Endosomal Escape for DNA Delivery
    Article Snippet: Dithiothreitol (DTT), Halt protease‐inhibitor‐cocktail, polyethyleneimine (PEI), imidazole, heparin agarose, KCl, Tris‐base, sodium dodecyl sulfate (SDS), 2‐mercaptoethanol, 4‐(2‐hydroxyethyl)‐1‐piperazineethanesulfonic acid (HEPES), agarose, kanamycin sulfate, glucose, Hoechst 33342, and Amicon Ultra‐15 PLGC Ultracel‐PL Membrane were purchased from Sigma‐Aldrich (St. Louis, MO, USA). .. Phosphate‐buffered saline (PBS) (1 mM KH 2 PO 4 , 155 mM NaCl, 3 mM Na 2 HPO 4 ), FBS, Dulbecco's Modified Eagle Medium (DMEM) GlutaMAX, penicillin‐streptomycin (pen‐strep), LP3000, CellMask Deep Red, NaCl, glycerol, bromphenol blue, ethylenediaminetetraacetic acid (EDTA), ZnCl 2 , FastDigest Buffer, FastDigest restriction enzymes and trypsin‐EDTA were obtained from Thermo Fisher Scientific (Waltham, MA, USA). .. Coomassie Brilliant Blue G‐250, CaCl 2 , paraformaldehyde (PFA) and bovine serum albumin (BSA) were purchased from Fluka‐Chemie AG (Buchs, Switzerland).

    Article Title: Large-Scale Functional Characterization of Low-Density Lipoprotein Receptor Gene Variants Improves Risk Assessment in Cardiovascular Disease.
    Article Snippet: .. Fluorescent low-density lipoprotein (DiI-LDL) uptake studies were performed similar to previous studies,15 with the following modifications: following 72 hours of overall treatment, DiI-LDL uptake was stimulated by adding 10 mg/mL DiI-LDL solution and incubated for 30 minutes at 37 C. Subsequently, cells were rinsed with phosphate-buffered saline, fixed with 4% paraformaldehyde, washed, and stained with Hoechst 33342 (5 mg/mL) and CellMask Deep Red (0.5 mg/mL) (Thermo Fisher Scientific). .. Images were acquired using a PerkinElmer OperaPhenix microscope equipped with 20 or 40 objectives.

    Modification:

    Article Title: Modular Calcium‐Responsive and CD9‐Targeted Phospholipase System Enhancing Endosomal Escape for DNA Delivery
    Article Snippet: Dithiothreitol (DTT), Halt protease‐inhibitor‐cocktail, polyethyleneimine (PEI), imidazole, heparin agarose, KCl, Tris‐base, sodium dodecyl sulfate (SDS), 2‐mercaptoethanol, 4‐(2‐hydroxyethyl)‐1‐piperazineethanesulfonic acid (HEPES), agarose, kanamycin sulfate, glucose, Hoechst 33342, and Amicon Ultra‐15 PLGC Ultracel‐PL Membrane were purchased from Sigma‐Aldrich (St. Louis, MO, USA). .. Phosphate‐buffered saline (PBS) (1 mM KH 2 PO 4 , 155 mM NaCl, 3 mM Na 2 HPO 4 ), FBS, Dulbecco's Modified Eagle Medium (DMEM) GlutaMAX, penicillin‐streptomycin (pen‐strep), LP3000, CellMask Deep Red, NaCl, glycerol, bromphenol blue, ethylenediaminetetraacetic acid (EDTA), ZnCl 2 , FastDigest Buffer, FastDigest restriction enzymes and trypsin‐EDTA were obtained from Thermo Fisher Scientific (Waltham, MA, USA). .. Coomassie Brilliant Blue G‐250, CaCl 2 , paraformaldehyde (PFA) and bovine serum albumin (BSA) were purchased from Fluka‐Chemie AG (Buchs, Switzerland).

    Incubation:

    Article Title: Large-Scale Functional Characterization of Low-Density Lipoprotein Receptor Gene Variants Improves Risk Assessment in Cardiovascular Disease.
    Article Snippet: .. Fluorescent low-density lipoprotein (DiI-LDL) uptake studies were performed similar to previous studies,15 with the following modifications: following 72 hours of overall treatment, DiI-LDL uptake was stimulated by adding 10 mg/mL DiI-LDL solution and incubated for 30 minutes at 37 C. Subsequently, cells were rinsed with phosphate-buffered saline, fixed with 4% paraformaldehyde, washed, and stained with Hoechst 33342 (5 mg/mL) and CellMask Deep Red (0.5 mg/mL) (Thermo Fisher Scientific). .. Images were acquired using a PerkinElmer OperaPhenix microscope equipped with 20 or 40 objectives.



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    a Cell viability was assessed by MTT assay 24 h post-treatment with 10 µM, 20 µM, and 25 µM DpPorA DE peptide. Data represents mean ± SEM from n = 4 (0 µM, 10 µM and 20 µM) and n = 3 (25 µM). Each dot represents a biological replicate, where each replicate was an independently seeded and treated culture of MDA-MB-231 cells on different days with different passage numbers. Statistical analysis was performed using one-way ANOVA with Dunnett’s multiple comparisons test comparing each treatment to the untreated control (0 µM); Adjusted p values correspond to *p = 0.0206 (10 µM), ***p = 0.0006 (20 µM), ****p < 0.0001 (25 µM) vs. 0 µM. b Immunofluorescence staining with <t>CellMask</t> <t>Deep</t> Red was used to evaluate the integrity of the cell membrane in Control, 0.00125% DDM and 25 µM DpPorA DE-treated cells. Cells were categorized into two phenotypes based on membrane integrity: Intact and Disrupted. Control cells displayed a mixture of phenotypes with a distribution of 67.38 % Intact and 32.62 % Disrupted. c DpPorA DE-treated cells showed a significant increase in the Disrupted phenotype, with 99.3% of cells being Disrupted, indicating a substantial impact of the DpPorA DE peptide on cell membrane integrity . d Fluorescence microscopy images showing different cell membrane integrity phenotypes in Control, 0.00125% DDM, and 25 µM DpPorA DE peptide-treated cells. The two different phenotypes are represented as a hashtag for Intact and the arrow indicates Disrupted cells (100 cells per group, scale bar 20 μm, magnification 63×). e Representative fluorescence images showing the distribution of 5-FAM-DpPorA DE (green) in MDA-MB-231 cells at 4 h and 24 h post-addition of peptide. Cell membranes were stained with CellMask (red) (50 cells per group, scale bar 20 μm, magnification 63×). f Single-cell fluorescence intensity scatter plot showing increased incorporation of 5-FAM-DpPorA DE at 24 h compared to 4 h, indicating time-dependent peptide incorporation in the cell population.
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    Image Search Results


    a Cell viability was assessed by MTT assay 24 h post-treatment with 10 µM, 20 µM, and 25 µM DpPorA DE peptide. Data represents mean ± SEM from n = 4 (0 µM, 10 µM and 20 µM) and n = 3 (25 µM). Each dot represents a biological replicate, where each replicate was an independently seeded and treated culture of MDA-MB-231 cells on different days with different passage numbers. Statistical analysis was performed using one-way ANOVA with Dunnett’s multiple comparisons test comparing each treatment to the untreated control (0 µM); Adjusted p values correspond to *p = 0.0206 (10 µM), ***p = 0.0006 (20 µM), ****p < 0.0001 (25 µM) vs. 0 µM. b Immunofluorescence staining with CellMask Deep Red was used to evaluate the integrity of the cell membrane in Control, 0.00125% DDM and 25 µM DpPorA DE-treated cells. Cells were categorized into two phenotypes based on membrane integrity: Intact and Disrupted. Control cells displayed a mixture of phenotypes with a distribution of 67.38 % Intact and 32.62 % Disrupted. c DpPorA DE-treated cells showed a significant increase in the Disrupted phenotype, with 99.3% of cells being Disrupted, indicating a substantial impact of the DpPorA DE peptide on cell membrane integrity . d Fluorescence microscopy images showing different cell membrane integrity phenotypes in Control, 0.00125% DDM, and 25 µM DpPorA DE peptide-treated cells. The two different phenotypes are represented as a hashtag for Intact and the arrow indicates Disrupted cells (100 cells per group, scale bar 20 μm, magnification 63×). e Representative fluorescence images showing the distribution of 5-FAM-DpPorA DE (green) in MDA-MB-231 cells at 4 h and 24 h post-addition of peptide. Cell membranes were stained with CellMask (red) (50 cells per group, scale bar 20 μm, magnification 63×). f Single-cell fluorescence intensity scatter plot showing increased incorporation of 5-FAM-DpPorA DE at 24 h compared to 4 h, indicating time-dependent peptide incorporation in the cell population.

    Journal: Nature Communications

    Article Title: Fabrication of cytotoxic mirror image nanopores

    doi: 10.1038/s41467-025-64025-6

    Figure Lengend Snippet: a Cell viability was assessed by MTT assay 24 h post-treatment with 10 µM, 20 µM, and 25 µM DpPorA DE peptide. Data represents mean ± SEM from n = 4 (0 µM, 10 µM and 20 µM) and n = 3 (25 µM). Each dot represents a biological replicate, where each replicate was an independently seeded and treated culture of MDA-MB-231 cells on different days with different passage numbers. Statistical analysis was performed using one-way ANOVA with Dunnett’s multiple comparisons test comparing each treatment to the untreated control (0 µM); Adjusted p values correspond to *p = 0.0206 (10 µM), ***p = 0.0006 (20 µM), ****p < 0.0001 (25 µM) vs. 0 µM. b Immunofluorescence staining with CellMask Deep Red was used to evaluate the integrity of the cell membrane in Control, 0.00125% DDM and 25 µM DpPorA DE-treated cells. Cells were categorized into two phenotypes based on membrane integrity: Intact and Disrupted. Control cells displayed a mixture of phenotypes with a distribution of 67.38 % Intact and 32.62 % Disrupted. c DpPorA DE-treated cells showed a significant increase in the Disrupted phenotype, with 99.3% of cells being Disrupted, indicating a substantial impact of the DpPorA DE peptide on cell membrane integrity . d Fluorescence microscopy images showing different cell membrane integrity phenotypes in Control, 0.00125% DDM, and 25 µM DpPorA DE peptide-treated cells. The two different phenotypes are represented as a hashtag for Intact and the arrow indicates Disrupted cells (100 cells per group, scale bar 20 μm, magnification 63×). e Representative fluorescence images showing the distribution of 5-FAM-DpPorA DE (green) in MDA-MB-231 cells at 4 h and 24 h post-addition of peptide. Cell membranes were stained with CellMask (red) (50 cells per group, scale bar 20 μm, magnification 63×). f Single-cell fluorescence intensity scatter plot showing increased incorporation of 5-FAM-DpPorA DE at 24 h compared to 4 h, indicating time-dependent peptide incorporation in the cell population.

    Article Snippet: After 24 h of treatment, cells were stained with CellMask Deep Red (Thermo Fisher Scientific, C10046 ; 1:4000 dilution in PBS) for 5 min at 37 °C.

    Techniques: MTT Assay, Control, Immunofluorescence, Staining, Membrane, Fluorescence, Microscopy