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cellmask deep red plasma membrane stain  (Thermo Fisher)


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    Thermo Fisher cellmask deep red plasma membrane stain
    Cellmask Deep Red Plasma Membrane Stain, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 91793 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cellmask deep red plasma membrane stain/product/Thermo Fisher
    Average 99 stars, based on 91793 article reviews
    cellmask deep red plasma membrane stain - by Bioz Stars, 2026-03
    99/100 stars

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    a Cell viability was assessed by MTT assay 24 h post-treatment with 10 µM, 20 µM, and 25 µM DpPorA DE peptide. Data represents mean ± SEM from n = 4 (0 µM, 10 µM and 20 µM) and n = 3 (25 µM). Each dot represents a biological replicate, where each replicate was an independently seeded and treated culture of MDA-MB-231 cells on different days with different passage numbers. Statistical analysis was performed using one-way ANOVA with Dunnett’s multiple comparisons test comparing each treatment to the untreated control (0 µM); Adjusted p values correspond to *p = 0.0206 (10 µM), ***p = 0.0006 (20 µM), ****p < 0.0001 (25 µM) vs. 0 µM. b Immunofluorescence staining with <t>CellMask</t> <t>Deep</t> Red was used to evaluate the integrity of the cell membrane in Control, 0.00125% DDM and 25 µM DpPorA DE-treated cells. Cells were categorized into two phenotypes based on membrane integrity: Intact and Disrupted. Control cells displayed a mixture of phenotypes with a distribution of 67.38 % Intact and 32.62 % Disrupted. c DpPorA DE-treated cells showed a significant increase in the Disrupted phenotype, with 99.3% of cells being Disrupted, indicating a substantial impact of the DpPorA DE peptide on cell membrane integrity . d Fluorescence microscopy images showing different cell membrane integrity phenotypes in Control, 0.00125% DDM, and 25 µM DpPorA DE peptide-treated cells. The two different phenotypes are represented as a hashtag for Intact and the arrow indicates Disrupted cells (100 cells per group, scale bar 20 μm, magnification 63×). e Representative fluorescence images showing the distribution of 5-FAM-DpPorA DE (green) in MDA-MB-231 cells at 4 h and 24 h post-addition of peptide. Cell membranes were stained with CellMask (red) (50 cells per group, scale bar 20 μm, magnification 63×). f Single-cell fluorescence intensity scatter plot showing increased incorporation of 5-FAM-DpPorA DE at 24 h compared to 4 h, indicating time-dependent peptide incorporation in the cell population.
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    a Cell viability was assessed by MTT assay 24 h post-treatment with 10 µM, 20 µM, and 25 µM DpPorA DE peptide. Data represents mean ± SEM from n = 4 (0 µM, 10 µM and 20 µM) and n = 3 (25 µM). Each dot represents a biological replicate, where each replicate was an independently seeded and treated culture of MDA-MB-231 cells on different days with different passage numbers. Statistical analysis was performed using one-way ANOVA with Dunnett’s multiple comparisons test comparing each treatment to the untreated control (0 µM); Adjusted p values correspond to *p = 0.0206 (10 µM), ***p = 0.0006 (20 µM), ****p < 0.0001 (25 µM) vs. 0 µM. b Immunofluorescence staining with <t>CellMask</t> <t>Deep</t> Red was used to evaluate the integrity of the cell membrane in Control, 0.00125% DDM and 25 µM DpPorA DE-treated cells. Cells were categorized into two phenotypes based on membrane integrity: Intact and Disrupted. Control cells displayed a mixture of phenotypes with a distribution of 67.38 % Intact and 32.62 % Disrupted. c DpPorA DE-treated cells showed a significant increase in the Disrupted phenotype, with 99.3% of cells being Disrupted, indicating a substantial impact of the DpPorA DE peptide on cell membrane integrity . d Fluorescence microscopy images showing different cell membrane integrity phenotypes in Control, 0.00125% DDM, and 25 µM DpPorA DE peptide-treated cells. The two different phenotypes are represented as a hashtag for Intact and the arrow indicates Disrupted cells (100 cells per group, scale bar 20 μm, magnification 63×). e Representative fluorescence images showing the distribution of 5-FAM-DpPorA DE (green) in MDA-MB-231 cells at 4 h and 24 h post-addition of peptide. Cell membranes were stained with CellMask (red) (50 cells per group, scale bar 20 μm, magnification 63×). f Single-cell fluorescence intensity scatter plot showing increased incorporation of 5-FAM-DpPorA DE at 24 h compared to 4 h, indicating time-dependent peptide incorporation in the cell population.
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    a Cell viability was assessed by MTT assay 24 h post-treatment with 10 µM, 20 µM, and 25 µM DpPorA DE peptide. Data represents mean ± SEM from n = 4 (0 µM, 10 µM and 20 µM) and n = 3 (25 µM). Each dot represents a biological replicate, where each replicate was an independently seeded and treated culture of MDA-MB-231 cells on different days with different passage numbers. Statistical analysis was performed using one-way ANOVA with Dunnett’s multiple comparisons test comparing each treatment to the untreated control (0 µM); Adjusted p values correspond to *p = 0.0206 (10 µM), ***p = 0.0006 (20 µM), ****p < 0.0001 (25 µM) vs. 0 µM. b Immunofluorescence staining with <t>CellMask</t> <t>Deep</t> Red was used to evaluate the integrity of the cell membrane in Control, 0.00125% DDM and 25 µM DpPorA DE-treated cells. Cells were categorized into two phenotypes based on membrane integrity: Intact and Disrupted. Control cells displayed a mixture of phenotypes with a distribution of 67.38 % Intact and 32.62 % Disrupted. c DpPorA DE-treated cells showed a significant increase in the Disrupted phenotype, with 99.3% of cells being Disrupted, indicating a substantial impact of the DpPorA DE peptide on cell membrane integrity . d Fluorescence microscopy images showing different cell membrane integrity phenotypes in Control, 0.00125% DDM, and 25 µM DpPorA DE peptide-treated cells. The two different phenotypes are represented as a hashtag for Intact and the arrow indicates Disrupted cells (100 cells per group, scale bar 20 μm, magnification 63×). e Representative fluorescence images showing the distribution of 5-FAM-DpPorA DE (green) in MDA-MB-231 cells at 4 h and 24 h post-addition of peptide. Cell membranes were stained with CellMask (red) (50 cells per group, scale bar 20 μm, magnification 63×). f Single-cell fluorescence intensity scatter plot showing increased incorporation of 5-FAM-DpPorA DE at 24 h compared to 4 h, indicating time-dependent peptide incorporation in the cell population.
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    a Cell viability was assessed by MTT assay 24 h post-treatment with 10 µM, 20 µM, and 25 µM DpPorA DE peptide. Data represents mean ± SEM from n = 4 (0 µM, 10 µM and 20 µM) and n = 3 (25 µM). Each dot represents a biological replicate, where each replicate was an independently seeded and treated culture of MDA-MB-231 cells on different days with different passage numbers. Statistical analysis was performed using one-way ANOVA with Dunnett’s multiple comparisons test comparing each treatment to the untreated control (0 µM); Adjusted p values correspond to *p = 0.0206 (10 µM), ***p = 0.0006 (20 µM), ****p < 0.0001 (25 µM) vs. 0 µM. b Immunofluorescence staining with <t>CellMask</t> <t>Deep</t> Red was used to evaluate the integrity of the cell membrane in Control, 0.00125% DDM and 25 µM DpPorA DE-treated cells. Cells were categorized into two phenotypes based on membrane integrity: Intact and Disrupted. Control cells displayed a mixture of phenotypes with a distribution of 67.38 % Intact and 32.62 % Disrupted. c DpPorA DE-treated cells showed a significant increase in the Disrupted phenotype, with 99.3% of cells being Disrupted, indicating a substantial impact of the DpPorA DE peptide on cell membrane integrity . d Fluorescence microscopy images showing different cell membrane integrity phenotypes in Control, 0.00125% DDM, and 25 µM DpPorA DE peptide-treated cells. The two different phenotypes are represented as a hashtag for Intact and the arrow indicates Disrupted cells (100 cells per group, scale bar 20 μm, magnification 63×). e Representative fluorescence images showing the distribution of 5-FAM-DpPorA DE (green) in MDA-MB-231 cells at 4 h and 24 h post-addition of peptide. Cell membranes were stained with CellMask (red) (50 cells per group, scale bar 20 μm, magnification 63×). f Single-cell fluorescence intensity scatter plot showing increased incorporation of 5-FAM-DpPorA DE at 24 h compared to 4 h, indicating time-dependent peptide incorporation in the cell population.
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    a Cell viability was assessed by MTT assay 24 h post-treatment with 10 µM, 20 µM, and 25 µM DpPorA DE peptide. Data represents mean ± SEM from n = 4 (0 µM, 10 µM and 20 µM) and n = 3 (25 µM). Each dot represents a biological replicate, where each replicate was an independently seeded and treated culture of MDA-MB-231 cells on different days with different passage numbers. Statistical analysis was performed using one-way ANOVA with Dunnett’s multiple comparisons test comparing each treatment to the untreated control (0 µM); Adjusted p values correspond to *p = 0.0206 (10 µM), ***p = 0.0006 (20 µM), ****p < 0.0001 (25 µM) vs. 0 µM. b Immunofluorescence staining with <t>CellMask</t> <t>Deep</t> Red was used to evaluate the integrity of the cell membrane in Control, 0.00125% DDM and 25 µM DpPorA DE-treated cells. Cells were categorized into two phenotypes based on membrane integrity: Intact and Disrupted. Control cells displayed a mixture of phenotypes with a distribution of 67.38 % Intact and 32.62 % Disrupted. c DpPorA DE-treated cells showed a significant increase in the Disrupted phenotype, with 99.3% of cells being Disrupted, indicating a substantial impact of the DpPorA DE peptide on cell membrane integrity . d Fluorescence microscopy images showing different cell membrane integrity phenotypes in Control, 0.00125% DDM, and 25 µM DpPorA DE peptide-treated cells. The two different phenotypes are represented as a hashtag for Intact and the arrow indicates Disrupted cells (100 cells per group, scale bar 20 μm, magnification 63×). e Representative fluorescence images showing the distribution of 5-FAM-DpPorA DE (green) in MDA-MB-231 cells at 4 h and 24 h post-addition of peptide. Cell membranes were stained with CellMask (red) (50 cells per group, scale bar 20 μm, magnification 63×). f Single-cell fluorescence intensity scatter plot showing increased incorporation of 5-FAM-DpPorA DE at 24 h compared to 4 h, indicating time-dependent peptide incorporation in the cell population.
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    a Cell viability was assessed by MTT assay 24 h post-treatment with 10 µM, 20 µM, and 25 µM DpPorA DE peptide. Data represents mean ± SEM from n = 4 (0 µM, 10 µM and 20 µM) and n = 3 (25 µM). Each dot represents a biological replicate, where each replicate was an independently seeded and treated culture of MDA-MB-231 cells on different days with different passage numbers. Statistical analysis was performed using one-way ANOVA with Dunnett’s multiple comparisons test comparing each treatment to the untreated control (0 µM); Adjusted p values correspond to *p = 0.0206 (10 µM), ***p = 0.0006 (20 µM), ****p < 0.0001 (25 µM) vs. 0 µM. b Immunofluorescence staining with <t>CellMask</t> <t>Deep</t> Red was used to evaluate the integrity of the cell membrane in Control, 0.00125% DDM and 25 µM DpPorA DE-treated cells. Cells were categorized into two phenotypes based on membrane integrity: Intact and Disrupted. Control cells displayed a mixture of phenotypes with a distribution of 67.38 % Intact and 32.62 % Disrupted. c DpPorA DE-treated cells showed a significant increase in the Disrupted phenotype, with 99.3% of cells being Disrupted, indicating a substantial impact of the DpPorA DE peptide on cell membrane integrity . d Fluorescence microscopy images showing different cell membrane integrity phenotypes in Control, 0.00125% DDM, and 25 µM DpPorA DE peptide-treated cells. The two different phenotypes are represented as a hashtag for Intact and the arrow indicates Disrupted cells (100 cells per group, scale bar 20 μm, magnification 63×). e Representative fluorescence images showing the distribution of 5-FAM-DpPorA DE (green) in MDA-MB-231 cells at 4 h and 24 h post-addition of peptide. Cell membranes were stained with CellMask (red) (50 cells per group, scale bar 20 μm, magnification 63×). f Single-cell fluorescence intensity scatter plot showing increased incorporation of 5-FAM-DpPorA DE at 24 h compared to 4 h, indicating time-dependent peptide incorporation in the cell population.
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    a Cell viability was assessed by MTT assay 24 h post-treatment with 10 µM, 20 µM, and 25 µM DpPorA DE peptide. Data represents mean ± SEM from n = 4 (0 µM, 10 µM and 20 µM) and n = 3 (25 µM). Each dot represents a biological replicate, where each replicate was an independently seeded and treated culture of MDA-MB-231 cells on different days with different passage numbers. Statistical analysis was performed using one-way ANOVA with Dunnett’s multiple comparisons test comparing each treatment to the untreated control (0 µM); Adjusted p values correspond to *p = 0.0206 (10 µM), ***p = 0.0006 (20 µM), ****p < 0.0001 (25 µM) vs. 0 µM. b Immunofluorescence staining with <t>CellMask</t> <t>Deep</t> Red was used to evaluate the integrity of the cell membrane in Control, 0.00125% DDM and 25 µM DpPorA DE-treated cells. Cells were categorized into two phenotypes based on membrane integrity: Intact and Disrupted. Control cells displayed a mixture of phenotypes with a distribution of 67.38 % Intact and 32.62 % Disrupted. c DpPorA DE-treated cells showed a significant increase in the Disrupted phenotype, with 99.3% of cells being Disrupted, indicating a substantial impact of the DpPorA DE peptide on cell membrane integrity . d Fluorescence microscopy images showing different cell membrane integrity phenotypes in Control, 0.00125% DDM, and 25 µM DpPorA DE peptide-treated cells. The two different phenotypes are represented as a hashtag for Intact and the arrow indicates Disrupted cells (100 cells per group, scale bar 20 μm, magnification 63×). e Representative fluorescence images showing the distribution of 5-FAM-DpPorA DE (green) in MDA-MB-231 cells at 4 h and 24 h post-addition of peptide. Cell membranes were stained with CellMask (red) (50 cells per group, scale bar 20 μm, magnification 63×). f Single-cell fluorescence intensity scatter plot showing increased incorporation of 5-FAM-DpPorA DE at 24 h compared to 4 h, indicating time-dependent peptide incorporation in the cell population.
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    a Cell viability was assessed by MTT assay 24 h post-treatment with 10 µM, 20 µM, and 25 µM DpPorA DE peptide. Data represents mean ± SEM from n = 4 (0 µM, 10 µM and 20 µM) and n = 3 (25 µM). Each dot represents a biological replicate, where each replicate was an independently seeded and treated culture of MDA-MB-231 cells on different days with different passage numbers. Statistical analysis was performed using one-way ANOVA with Dunnett’s multiple comparisons test comparing each treatment to the untreated control (0 µM); Adjusted p values correspond to *p = 0.0206 (10 µM), ***p = 0.0006 (20 µM), ****p < 0.0001 (25 µM) vs. 0 µM. b Immunofluorescence staining with CellMask Deep Red was used to evaluate the integrity of the cell membrane in Control, 0.00125% DDM and 25 µM DpPorA DE-treated cells. Cells were categorized into two phenotypes based on membrane integrity: Intact and Disrupted. Control cells displayed a mixture of phenotypes with a distribution of 67.38 % Intact and 32.62 % Disrupted. c DpPorA DE-treated cells showed a significant increase in the Disrupted phenotype, with 99.3% of cells being Disrupted, indicating a substantial impact of the DpPorA DE peptide on cell membrane integrity . d Fluorescence microscopy images showing different cell membrane integrity phenotypes in Control, 0.00125% DDM, and 25 µM DpPorA DE peptide-treated cells. The two different phenotypes are represented as a hashtag for Intact and the arrow indicates Disrupted cells (100 cells per group, scale bar 20 μm, magnification 63×). e Representative fluorescence images showing the distribution of 5-FAM-DpPorA DE (green) in MDA-MB-231 cells at 4 h and 24 h post-addition of peptide. Cell membranes were stained with CellMask (red) (50 cells per group, scale bar 20 μm, magnification 63×). f Single-cell fluorescence intensity scatter plot showing increased incorporation of 5-FAM-DpPorA DE at 24 h compared to 4 h, indicating time-dependent peptide incorporation in the cell population.

    Journal: Nature Communications

    Article Title: Fabrication of cytotoxic mirror image nanopores

    doi: 10.1038/s41467-025-64025-6

    Figure Lengend Snippet: a Cell viability was assessed by MTT assay 24 h post-treatment with 10 µM, 20 µM, and 25 µM DpPorA DE peptide. Data represents mean ± SEM from n = 4 (0 µM, 10 µM and 20 µM) and n = 3 (25 µM). Each dot represents a biological replicate, where each replicate was an independently seeded and treated culture of MDA-MB-231 cells on different days with different passage numbers. Statistical analysis was performed using one-way ANOVA with Dunnett’s multiple comparisons test comparing each treatment to the untreated control (0 µM); Adjusted p values correspond to *p = 0.0206 (10 µM), ***p = 0.0006 (20 µM), ****p < 0.0001 (25 µM) vs. 0 µM. b Immunofluorescence staining with CellMask Deep Red was used to evaluate the integrity of the cell membrane in Control, 0.00125% DDM and 25 µM DpPorA DE-treated cells. Cells were categorized into two phenotypes based on membrane integrity: Intact and Disrupted. Control cells displayed a mixture of phenotypes with a distribution of 67.38 % Intact and 32.62 % Disrupted. c DpPorA DE-treated cells showed a significant increase in the Disrupted phenotype, with 99.3% of cells being Disrupted, indicating a substantial impact of the DpPorA DE peptide on cell membrane integrity . d Fluorescence microscopy images showing different cell membrane integrity phenotypes in Control, 0.00125% DDM, and 25 µM DpPorA DE peptide-treated cells. The two different phenotypes are represented as a hashtag for Intact and the arrow indicates Disrupted cells (100 cells per group, scale bar 20 μm, magnification 63×). e Representative fluorescence images showing the distribution of 5-FAM-DpPorA DE (green) in MDA-MB-231 cells at 4 h and 24 h post-addition of peptide. Cell membranes were stained with CellMask (red) (50 cells per group, scale bar 20 μm, magnification 63×). f Single-cell fluorescence intensity scatter plot showing increased incorporation of 5-FAM-DpPorA DE at 24 h compared to 4 h, indicating time-dependent peptide incorporation in the cell population.

    Article Snippet: After 24 h of treatment, cells were stained with CellMask Deep Red (Thermo Fisher Scientific, C10046 ; 1:4000 dilution in PBS) for 5 min at 37 °C.

    Techniques: MTT Assay, Control, Immunofluorescence, Staining, Membrane, Fluorescence, Microscopy